anti rack1 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti rack1
Anti Rack1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+rack1+antibody/pm41634833-89-21-22
Average 86 stars, based on 1 article reviews
Anti Rack1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+rack1+antibody/pm41634833-89-21-22
Average 86 stars, based on 1 article reviews
anti rack1 - by Bioz Stars,
2026-10
86/100 stars
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Nucleic Acid Electrophoresis:Article Title: RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer Article Snippet: .. Total protein lysates (20 μ g) were fractionated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to PVDF membranes, and blocked at 4°C for 4 h with 5% nonfat dry milk in Tris-buffered saline (pH 7.5) supplemented with 0.1% Tween 20, followed by overnight incubation with the Saline:Article Title: RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer Article Snippet: .. Total protein lysates (20 μ g) were fractionated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to PVDF membranes, and blocked at 4°C for 4 h with 5% nonfat dry milk in Tris-buffered saline (pH 7.5) supplemented with 0.1% Tween 20, followed by overnight incubation with the Incubation:Article Title: RACK1 Acts as a Potential Tumor Promoter in Colorectal Cancer Article Snippet: .. Total protein lysates (20 μ g) were fractionated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to PVDF membranes, and blocked at 4°C for 4 h with 5% nonfat dry milk in Tris-buffered saline (pH 7.5) supplemented with 0.1% Tween 20, followed by overnight incubation with the Article Title: Role of RACK1 on cell proliferation, adhesion, and bortezomib-induced apoptosis in multiple myeloma. Article Snippet: Receptor for activated C kinase 1 (RACK1), a scaffold protein, plays a crucial role in the progression of various cancers.. However, the biological function and underlying mechanism of RACK1 in multiple myeloma (MM) cells remain unclear.. The present study aimed to explore the function of RACK1 on the cell proliferation, adhesion, and bortezomib-induced apoptosis in MM. Article Title: SMYD3 synergises with RACK1 to promote colorectal cancer lung metastasis by recruiting SMAD3 Article Snippet: Lysis buffer (50 mM Tris base, 150 mM NaCl, 2 mM EDTA, 1% NP40), supplemented with protease and phosphatase inhibitors (NCM Biotech, China, #P002), was added to CRC cells, and protein samples were obtained by centrifugation to remove insoluble precipitates. .. Protein A/G magnetic beads were incubated with anti-Flag (CST, #14793S), anti-SMAD3 antibody (CST, #9523S, 1:100), Article Title: RACK1 is indispensable for porcine reproductive and respiratory syndrome virus replication and NF-κB activation in Marc-145 cells Article Snippet: .. Viral N protein or cellular proteins were probed by overnight incubation at 4 °C with rocking, with the primary antibodies, which was diluted in filtered 5% milk–PBST at a ratio of 1:500 (anti-N protein monoclonal antibody, VMRD, Cat. #080728–004, mouse origin), 1:1000 (anti- GAPDH polyclonal antibody, Proteintech, Cat. #20536-1-AP, rabbit origin), 1:1000 (anti-p65 antibody, Lsbio, Cat. #LS-C352792, rabbit origin), 1:1000 (anti-phospho-p65 Ser536 antibody, Cell Signaling Technology, Cat. #3033 s, rabbit origin), 1:1000 ( Magnetic Beads:Article Title: SMYD3 synergises with RACK1 to promote colorectal cancer lung metastasis by recruiting SMAD3 Article Snippet: Lysis buffer (50 mM Tris base, 150 mM NaCl, 2 mM EDTA, 1% NP40), supplemented with protease and phosphatase inhibitors (NCM Biotech, China, #P002), was added to CRC cells, and protein samples were obtained by centrifugation to remove insoluble precipitates. .. Protein A/G magnetic beads were incubated with anti-Flag (CST, #14793S), anti-SMAD3 antibody (CST, #9523S, 1:100), Concentration Assay:Article Title: Overexpression of RACK1 enhanced the replication of porcine reproductive and respiratory syndrome virus in Marc-145 cells and promoted the NF-κB activation via upregulating the expression and phosphorylation of TRAF2. Article Snippet: Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative pathogen for porcine reproductive and respiratory syndrome (PRRS), which lead to huge loss to porcine industry.. RACK1 (receptor of activated protein C kinase 1) was first identified as a receptor for protein kinase C. Mounting evidence demonstrated that RACK1 played diverse roles in NF-κB activation and virus infections.. We previously reported that siRNA knockdown of RACK1 inhibited PRRSV replication in Marc-145 cells, abrogated NF-κB activation induced by PRRSV infection and reduced the viral titer. other:Article Title: SMYD3 synergises with RACK1 to promote colorectal cancer lung metastasis by recruiting SMAD3. Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS mice injected with HCT116 mock and HCT116-shRACK1 cells were selected after tail vein injection and treated with BCI-121 (Selleck, #432529-82-3) at a dose of 5 mg/kg via peritoneal injection (i.p.) three times per week for five weeks. |
